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atf6  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc atf6
    CFZ enhances the pro-paraptotic effect of 125 I seed radiation in ESCC cells. (A) CFZ aggravated 125 I seed radiation-induced cytoplasmic vacuolation in Kyse-150 and EC-109 cells. Cells were treated with CFZ (20 nM, 24 h), 125 I seed radiation (6 Gy), and CHX (10 μM, 24 h) alone or in combination. Cell morphological changes were observed under light microscopy, and the percentage of vacuolated cells was measured. Scale bar (upper) = 100 μm, Scale bar (lower) = 30 μm. N = 6. (B and C) Combined treatment of 125 I seed radiation and CFZ increased cytosolic and mitochondrial Ca 2+ levels in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination, then stained with Fluo-3 AM or Rhod-2 AM and quantitatively analyzed by mean fluorescence intensity using flow cytometry. (D) CFZ aggravated 125 I seed radiation-induced ubiquitination, ER stress, and UPR in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination. Protein levels of Ub, β-actin (control for loading), Bip, p-eIf2α, IRE1α, <t>ATF6,</t> CHOP, and β-tubulin (control for loading) were analyzed by Western blot. (E) Combined treatment of 125 I seed radiation and CFZ did not influence the protein expression of Alix as analyzed by Western blot. N = 3, * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to control group. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared between indicated groups.
    Atf6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atf6/product/Cell Signaling Technology Inc
    Average 96 stars, based on 354 article reviews
    atf6 - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Carfilzomib promotes Iodine-125 seed radiation-induced apoptosis, paraptosis, and ferroptosis in esophageal squamous cell carcinoma by aggravating endoplasmic reticulum stress"

    Article Title: Carfilzomib promotes Iodine-125 seed radiation-induced apoptosis, paraptosis, and ferroptosis in esophageal squamous cell carcinoma by aggravating endoplasmic reticulum stress

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2025.102393

    CFZ enhances the pro-paraptotic effect of 125 I seed radiation in ESCC cells. (A) CFZ aggravated 125 I seed radiation-induced cytoplasmic vacuolation in Kyse-150 and EC-109 cells. Cells were treated with CFZ (20 nM, 24 h), 125 I seed radiation (6 Gy), and CHX (10 μM, 24 h) alone or in combination. Cell morphological changes were observed under light microscopy, and the percentage of vacuolated cells was measured. Scale bar (upper) = 100 μm, Scale bar (lower) = 30 μm. N = 6. (B and C) Combined treatment of 125 I seed radiation and CFZ increased cytosolic and mitochondrial Ca 2+ levels in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination, then stained with Fluo-3 AM or Rhod-2 AM and quantitatively analyzed by mean fluorescence intensity using flow cytometry. (D) CFZ aggravated 125 I seed radiation-induced ubiquitination, ER stress, and UPR in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination. Protein levels of Ub, β-actin (control for loading), Bip, p-eIf2α, IRE1α, ATF6, CHOP, and β-tubulin (control for loading) were analyzed by Western blot. (E) Combined treatment of 125 I seed radiation and CFZ did not influence the protein expression of Alix as analyzed by Western blot. N = 3, * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to control group. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared between indicated groups.
    Figure Legend Snippet: CFZ enhances the pro-paraptotic effect of 125 I seed radiation in ESCC cells. (A) CFZ aggravated 125 I seed radiation-induced cytoplasmic vacuolation in Kyse-150 and EC-109 cells. Cells were treated with CFZ (20 nM, 24 h), 125 I seed radiation (6 Gy), and CHX (10 μM, 24 h) alone or in combination. Cell morphological changes were observed under light microscopy, and the percentage of vacuolated cells was measured. Scale bar (upper) = 100 μm, Scale bar (lower) = 30 μm. N = 6. (B and C) Combined treatment of 125 I seed radiation and CFZ increased cytosolic and mitochondrial Ca 2+ levels in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination, then stained with Fluo-3 AM or Rhod-2 AM and quantitatively analyzed by mean fluorescence intensity using flow cytometry. (D) CFZ aggravated 125 I seed radiation-induced ubiquitination, ER stress, and UPR in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination. Protein levels of Ub, β-actin (control for loading), Bip, p-eIf2α, IRE1α, ATF6, CHOP, and β-tubulin (control for loading) were analyzed by Western blot. (E) Combined treatment of 125 I seed radiation and CFZ did not influence the protein expression of Alix as analyzed by Western blot. N = 3, * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to control group. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared between indicated groups.

    Techniques Used: Light Microscopy, Staining, Fluorescence, Flow Cytometry, Ubiquitin Proteomics, Control, Western Blot, Expressing



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    CFZ enhances the pro-paraptotic effect of 125 I seed radiation in ESCC cells. (A) CFZ aggravated 125 I seed radiation-induced cytoplasmic vacuolation in Kyse-150 and EC-109 cells. Cells were treated with CFZ (20 nM, 24 h), 125 I seed radiation (6 Gy), and CHX (10 μM, 24 h) alone or in combination. Cell morphological changes were observed under light microscopy, and the percentage of vacuolated cells was measured. Scale bar (upper) = 100 μm, Scale bar (lower) = 30 μm. N = 6. (B and C) Combined treatment of 125 I seed radiation and CFZ increased cytosolic and mitochondrial Ca 2+ levels in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination, then stained with Fluo-3 AM or Rhod-2 AM and quantitatively analyzed by mean fluorescence intensity using flow cytometry. (D) CFZ aggravated 125 I seed radiation-induced ubiquitination, ER stress, and UPR in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination. Protein levels of Ub, β-actin (control for loading), Bip, p-eIf2α, IRE1α, <t>ATF6,</t> CHOP, and β-tubulin (control for loading) were analyzed by Western blot. (E) Combined treatment of 125 I seed radiation and CFZ did not influence the protein expression of Alix as analyzed by Western blot. N = 3, * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to control group. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared between indicated groups.
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    Image Search Results


    CFZ enhances the pro-paraptotic effect of 125 I seed radiation in ESCC cells. (A) CFZ aggravated 125 I seed radiation-induced cytoplasmic vacuolation in Kyse-150 and EC-109 cells. Cells were treated with CFZ (20 nM, 24 h), 125 I seed radiation (6 Gy), and CHX (10 μM, 24 h) alone or in combination. Cell morphological changes were observed under light microscopy, and the percentage of vacuolated cells was measured. Scale bar (upper) = 100 μm, Scale bar (lower) = 30 μm. N = 6. (B and C) Combined treatment of 125 I seed radiation and CFZ increased cytosolic and mitochondrial Ca 2+ levels in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination, then stained with Fluo-3 AM or Rhod-2 AM and quantitatively analyzed by mean fluorescence intensity using flow cytometry. (D) CFZ aggravated 125 I seed radiation-induced ubiquitination, ER stress, and UPR in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination. Protein levels of Ub, β-actin (control for loading), Bip, p-eIf2α, IRE1α, ATF6, CHOP, and β-tubulin (control for loading) were analyzed by Western blot. (E) Combined treatment of 125 I seed radiation and CFZ did not influence the protein expression of Alix as analyzed by Western blot. N = 3, * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to control group. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared between indicated groups.

    Journal: Translational Oncology

    Article Title: Carfilzomib promotes Iodine-125 seed radiation-induced apoptosis, paraptosis, and ferroptosis in esophageal squamous cell carcinoma by aggravating endoplasmic reticulum stress

    doi: 10.1016/j.tranon.2025.102393

    Figure Lengend Snippet: CFZ enhances the pro-paraptotic effect of 125 I seed radiation in ESCC cells. (A) CFZ aggravated 125 I seed radiation-induced cytoplasmic vacuolation in Kyse-150 and EC-109 cells. Cells were treated with CFZ (20 nM, 24 h), 125 I seed radiation (6 Gy), and CHX (10 μM, 24 h) alone or in combination. Cell morphological changes were observed under light microscopy, and the percentage of vacuolated cells was measured. Scale bar (upper) = 100 μm, Scale bar (lower) = 30 μm. N = 6. (B and C) Combined treatment of 125 I seed radiation and CFZ increased cytosolic and mitochondrial Ca 2+ levels in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination, then stained with Fluo-3 AM or Rhod-2 AM and quantitatively analyzed by mean fluorescence intensity using flow cytometry. (D) CFZ aggravated 125 I seed radiation-induced ubiquitination, ER stress, and UPR in Kyse-150 and EC-109 cells. Cells were treated with 125 I seed radiation (6 Gy) and CFZ (20 nM, 24 h) alone or in combination. Protein levels of Ub, β-actin (control for loading), Bip, p-eIf2α, IRE1α, ATF6, CHOP, and β-tubulin (control for loading) were analyzed by Western blot. (E) Combined treatment of 125 I seed radiation and CFZ did not influence the protein expression of Alix as analyzed by Western blot. N = 3, * P < 0.05, ** P < 0.01, and *** P < 0.001 compared to control group. # P < 0.05, ## P < 0.01, and ### P < 0.001 compared between indicated groups.

    Article Snippet: Antibodies against p-H2AX (#9718), PARP (#9532), p53 (#2524), p-p53 (#9284), ubiquitin (Ub, #3936), p-eIF2α (#3398), IRE1α (#3294), ATF6 (#65,880), Alix (#92,880), LC3B (#3868), β-tubulin (#2128), β-actin (#4970) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Light Microscopy, Staining, Fluorescence, Flow Cytometry, Ubiquitin Proteomics, Control, Western Blot, Expressing